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CAPRIN1 Antibody

CAPRIN1 Antibody

ArtNr	PRS-43-648-0.1mg
Hersteller	ProSci
Menge	0.1 mg
Kategorie	Antibodies Western Blot ELISA Primary Antibodies
Typ	Antibody Polyclonal
Format	Liquid
Applikationen	WB, IHC, ELISA
Specific against	Human
Host	Goat
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	CAPRIN1, cell cycle associated protein 1, GPIAP1, GPIP137, M11S1, RNG105, p137GPI, GPI-anchored membrane protein 1, GPI-anchored protein p137, GPI-p137, RNA granule protein 105, activation/proliferation-associated protein 1, caprin 1, caprin-1, cell cycle
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Shipping	blue ice or RT
By Research Area	Immunology, Signal Transduction,
Homology	Expected Species Reactivity based on sequence homology: Rat, Dog, Pig
Immunogen	The immunogen for this antibody is: C-EQRPQKEPIDQ
Applications	Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 80+140kDa bands observed in lysates of cell line Caco-2, HeLa, Jurkat and K562 (calculated MW of 78.4kDa according to NP_005889.3). The ~140kDa band corresponds to the reported p137 (Ellis and Luzio, J Biol Chem. 1995 Sep 1; 270(35):20717-23.PMID: 7657653). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 5ug/ml.
Specificity	This antibody is expected to recognize both reported isoforms (NP_005889.3; NP_976240.1).
Predicted Molecular Weight	Approx 80 + 140kDa
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality	Polyclonal
Conjugate	Unconjugated
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Storage Conditions	Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products	Cat. No. 43-648P - CAPRIN1 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	CAPRIN1
Accession #	NP_005889.3, NP_976240.1
Protein Gi #	42558250
Ncbi Gene Id #	4076
Ncbi Official Symbol	CAPRIN1
Ncbi Official Full Name	cell cycle associated protein 1
Ncbi Organism	Homo sapiens
Swissprot #	Q14444
Background References 1	Wang B, David MD, Schrader JW.Absence of caprin-1 results in defects in cellular proliferation.
1st Image Caption	43-648 (0.3ug/ml) staining of Jurkat (A) and HeLa (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
2nd Image Caption	43-648 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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Menge: 0.1 mg

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