ArtNr |
PRS-4291-0.1mg |
Hersteller |
ProSci
|
Menge |
0.1 mg |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, IF, ICC, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
KIF5 Antibody: NKHC, MY050, SPG10, D12S1889, NKHC1, Kinesin heavy chain isoform 5A, Kinesin heavy chain neuron-specific 1, NKHC |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Neuroscience |
Immunogen |
KIF5 antibody was raised against a 20 amino acid synthetic peptide from near the center of human KIF5.
The immunogen is located within amino acids 330 - 380 of KIF5. |
Applications |
KIF5 antibody can be used for detection of KIF5 by Western blot at 0.5 - 1 μ g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Specificity |
KIF5 has at least three known isoforms; this antibody recognizes all three. |
Positive Control 1 |
Cat. No. 1204 - K562 Cell Lysate |
Positive Control 2 |
Cat. No. 17-004 - K-562 Cell Slide |
Predicted Molecular Weight |
Predicted: 105, 114 kDa
Observed: 118 kDa |
Purification |
KIF5 Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
KIF5 Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
KIF5 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 4291P – KIF5 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
KIF5A |
Accession # |
Q12840 |
Protein Gi # |
143811412 |
Ncbi Gene Id # |
3798 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
KIF5A |
Ncbi Official Full Name |
kinesin family member 5A |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q12840 |
Background |
KIF5 Antibody: The kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. Mutations in one of the three isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias. |
Background References 1 |
Hirokawa N. mRNA transport in dendrites: RNA granules, motors and tracks. J. Neurosci. 2006; 26:7139-42. |
Background References 2 |
Niclas J, Navone F, Hom-Booher N, et al. Cloning and localization of a conventional KIF5 motor expressed exclusively in neurons. Neuron 1994; 12:1059-72. |
Background References 3 |
Fichera M, Lo Giudice M, Falco M, et al. Evidence of kinesin heavy chain (KIF5A) involvement in pure hereditary spastic paraplegia. Neurology 2004; 63:1108-10. |
1st Image Caption |
Western blot analysis of KIF5 in K562 cell lysate with KIF5 antibody at (A) 0.5 and (B) 1 μ g/mL. |
2nd Image Caption |
Immunocytochemistry of KIF5 in K562 cells with KIF5 antibody at 5 μ g/mL. |
3rd Image Caption |
Immunofluorescence of KIF5 in K562 cells with KIF5 antibody at 20 μ g/mL. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.