ORAI3 Antibody

Immunology

ORAI3 antibody can be used for detection of ORAI3 by Western blot at 2 - 4 μ g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ g/mL. For immunofluorescence start at 10 μ g/mL.

Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Cat. No. 1288 - A20 Cell Lysate

Predicted: 32 kDa

Observed: 34 kDa

Polyclonal

Unconjugated

1 mg/mL

Cat. No. 4215P – ORAI3 Peptide

None

Q9BRQ5

93129

ORAI3

Homo sapiens

ORAI3 Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter.

Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.

Mercer JC, DeHaven WI, Smyth JT, et al. Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1. J. Biol. Chem. 2006; 281:24979-90.

Immunocytochemistry of ORAI3 in A20 cells with ORAI3 antibody at 5 μ g/mL.