ArtNr |
PRS-4125-0.1mg |
Hersteller |
ProSci
|
Menge |
0.1 mg |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
STIM2 Antibody: KIAA1482, Stromal interaction molecule 2 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Immunology |
Immunogen |
STIM2 antibody was raised against a 17 amino acid synthetic peptide from near the center of human STIM2.
The immunogen is located within amino acids 140 - 190 of STIM2. |
Applications |
STIM2 antibody can be used for detection of STIM2 by Western blot at 0.5 - 2 μ g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Positive Control 1 |
Cat. No. 1288 - A20 Cell Lysate |
Positive Control 2 |
Cat. No. 1306 - Human Spleen Tissue Lysate |
Positive Control 3 |
Cat. No. 10-901 - Human Spleen Tissue Slide |
Purification |
STIM2 Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
STIM2 Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
STIM2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 4125P – STIM2 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
STIM2 |
Accession # |
NP_065911 |
Protein Gi # |
41349446 |
Ncbi Gene Id # |
57620 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
STIM2 |
Ncbi Official Full Name |
stromal interaction molecule 2 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9P246 |
Background |
STIM2 Antibody: In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++-release-activated Ca++ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca++ entry. At least three isoforms of STIM2 are known to exist. This STIM2 antibody is predicted to have no cross-reactivity to STIM1. |
Background References 1 |
Luik RM and Lewis RS. New insights into the molecular mechanisms of store-operated Ca2+ signaling in T cells. Trends Mol. Med.2007; 13:103-7. |
Background References 2 |
Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature2006; 441:179-85. |
Background References 3 |
Zhang SL, Yu Y, Roos J, et al. STIM1 is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane. Nature2005; 437:902-5. |
Background References 4 |
Spassova MA, Soboloff J, He L-P, et al. STIM1 has a plasma membrane role in the activation of store-operated Ca2+ channels. Proc. Natl. Acad. Sci. USA2006; 103:4040-5. |
1st Image Caption |
Western blot analysis of STIM2 in A-20 cell lysate with STIM2 antibody at (A) 0.5, (B) 1 and (C) 2 μ g/mL. |
2nd Image Caption |
Immunohistochemistry of STIM2 in human spleen tissue with STIM2 antibody at 2.5 μ g/mL. |
3rd Image Caption |
Immunofluorescence of STIM2 in Human Spleen cells with STIM2 antibody at 20 μ g/mL. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.