NOV Antibody

Antibody Pairs

ELISA:
Indirect:
To detect hNOV by indirect ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNOV.

Sandwich
To detect hNOV by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human NOV as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNOV.

Western Blot:
To detect hNOV by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNOV is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Unconjugated

This product is for research use only.

NOV

1352515

Centrifuge vial prior to opening.

nephroblastoma overexpressed

P48745

To detect hNOV by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNOV is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.25 ug/ml-0.50 ug/ml with an overnight incubation at 4˚ C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.