ArtNr |
PRS-38-183-0.1mg |
Hersteller |
ProSci
|
Menge |
0.1 mg |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Lyophilized |
Applikationen |
WB, ELISA |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
OL-64Serpin A12, OL-64, Vaspin |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Growth Factor, Signal Transduction, Neuroscience, Antibody Pairs |
Immunogen |
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hVaspin. Human Vaspin specific antibody was purified by affinity chromatography employing immobilized hVaspin matrix |
Applications |
ELISA: Indirect: To detect hVaspin by indirect ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVaspin.
Sandwich To detect hVaspin by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Vaspin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVaspin.
Western Blot: To detect hVaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Storage Conditions |
Vaspin antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
SERPINA12 |
Accession # |
Q8IW75 |
Protein Gi # |
74728144 |
Ncbi Gene Id # |
145264 |
User Note |
Centrifuge vial prior to opening. |
Ncbi Official Symbol |
SERPINA12 |
Ncbi Official Full Name |
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q8IW75 |
Background |
Vaspin (serpina12) is a adipocytokine expressed predominantly in visceral white adipose tissues. It has three beta sheets, nine alpha helices, and one central loop, which are distinctive structural features of Serpin family members. The |
1st Image Caption |
To detect hVaspin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Vaspin (38-184) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVaspin. |
2nd Image Caption |
To detect hVaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
3rd Image Caption |
To detect hVaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.