ArtNr |
PRS-38-137-0.1mg |
Hersteller |
ProSci
|
Menge |
0.1 mg |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Lyophilized |
Applikationen |
WB, ELISA |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
FSFollistatin, Activin-binding protein, FS |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Signal Transduction, Growth Factor, Antibody Pairs, Functional Assays |
Immunogen |
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFollistatin. Human Follistatin specific antibody was purified by affinity chromatography employing immobilized hFollistatin matrix. |
Applications |
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human Follistatin (100.0 ng/mL), a concentration of 8.0-12.0 μ g/mL of this antibody is required.
ELISA: Indirect: To detect hFollistatin by indirect ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFollistatin.
Sandwich To detect hFollistatin by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Follistatin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFollistatin.
Western Blot: To detect hFollistatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFollistatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Storage Conditions |
Follistatin antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
FST |
Accession # |
P19883 |
Protein Gi # |
23831079 |
Ncbi Gene Id # |
10468 |
User Note |
Centrifuge vial prior to opening. |
Ncbi Official Symbol |
FST |
Ncbi Official Full Name |
follistatin |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P19883 |
Background |
Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. There is evidence linking polycystic ovary syndrome and follistatin. Follistatin binds directly to activin and functions as an activin antagonist. It specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone |
1st Image Caption |
To detect Human Follistatin by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Follistatin (38-138) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Follistatin. |
2nd Image Caption |
To detect Human Follistatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human Follistatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
3rd Image Caption |
To detect Human Follistatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human Follistatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.
Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.