LSD1 Antibody

blue ice or RT

Anti-LSD1 antibody (3761) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human LSD1.

The immunogen is located within amino acids 40-90 of LSD1.

Cat. No. 1286 - P815 Cell Lysate

Cat. No. 1205 - Jurkat Cell Lysate

Predicted: 93kD

Observed: 110 kD

Human LSD1 has 2 isoforms, including isoform 1 (852aa, 93kD) and isoform 2 (876aa, 95kD). Mouse LSD1 has one isoform (853aa, 93kD) and Rat LSD1 also has one isoform (872aa, 95kD). 3761 can detect all isoforms of human, mouse and rat.

Polyclonal

Unconjugated

1 mg/mL

Cat. No. 3761P – LSD1 Peptide

None

NP_001009999

23028

KDM1

Homo sapiens

LSD1 Antibody: Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction. Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription. LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation. Experiments have shown that CoREST, a SANT domain-containing corepressor acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein, inhibits CoREST/LSD1 activity in vitro. LSD1-mediated histone demethylation thus may have significant effects on gene expression.

Kouzarides et al. Curr. Opin. Genet. Dev. 2002; 12:198-209.

Nakamura et al. Mol. Cell 2002; 10:1119-28.

Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 μ g of lysates per lane.Antibodies: LSD1 3761 (0.2 μ g/mL), LSD1 14-050 (1 μ g/mL), beta-actin 3779 (1 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 4 Western Blot Validation in Mouse P815 Cell Lines
Loading: 15 μ g of lysates per lane.Antibodies: LSD1 3761, (A: 1 μ g/mL, B: 2 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 6 Immunofluorescence Validation of LSD1 in human breast carcinoma Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed human breast carcinoma Tissue labeling LSD1 with 3761 at 20 μ g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green/blue).