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XBP-1 Antibody

XBP-1 Antibody

ArtNr	PRS-3687-0.02mg
Hersteller	ProSci
Menge	0.02 mg
Quantity options	0.02 mg 0.1 mg
Kategorie	Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Immunocytochemistry Primary Antibodies
Typ	Antibody Polyclonal
Format	Liquid
Applikationen	WB, IF, ICC, ELISA, IHC-P
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Konjugat/Tag	Unconjugated
Citations	Kammoun et al. GRP78 expression inhibits insulin and ER stress-induced SREBP-1c activation and reduces hepatic steatosis in mice. J Clin Invest. 2009;119(5):1201-15. PMID: PMID:19363290 Lerat et al. Hepatitis C virus proteins induce lipogenesis and defective triglyceride secretion in transgenic mice. J Biol Chem. 2009;284(48):33466-74. PMID: PMID: 19808675 Yoshida et al. Cell 2001; 107:881-91. Calfon et al. Nature 2002; 415:92-6. Haze et al. Mol. Cell. Biol. 1999; 10:3787-99. Little et al. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18.
NCBI	XBP1
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	XBP-1 Antibody: XBP2, TREB5, XBP-1, XBP2, X-box-binding protein 1, Tax-responsive element-binding protein 5
Lieferbar
Manufacturer - Type	Primary Antibodies
Manufacturer - Applications	WB: 0.25-2 μg/mL; IF: 20 μg/mL; ICC: 10 μg/mL; IHC: 2-5 μg/mL. Antibody validated: Western Blot in human samples; Immunofluorescence in human and rat samples; Immunocytochemistry in human samples; Immunohistochemistry in human, mouse, and rat samples. All other applications and species not yet tested.
Country of Origin	USA
Shipping Temperature	Blue ice
Storage Conditions	XBP-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight	Predicted: 28/40kD Observed: 28/40 kD
Manufacturer - Research Area	Signal Transduction, Chemokines & Cytokines
Background	XBP-1 Antibody: X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.
Badges	Trial Size, Most Images, KO
Protein Gi #	60416406
Purification	XBP-1 Antibody is affinity chromatography purified via peptide column.
Isoforms	Human XBP-1 has two isoforms, including isoform 1 (261aa, 29kD) and isoform 2 (376aa, 40kD). Mouse XBP-1 also has two isoforms, including isoform 1 (267aa, 29.6kD) and isoform 2 (371aa, 40kD). And Rat XBP-1 has two isoforms as well, including isoform 1 (267aa, 30kD) and isoform 2 (371aa, 40kD). 3687 can detect human, mouse and rat.
Positive Control 1	PRS-1414-0.1mg
Positive Control 2	PRS-1464-0.1mg
Positive Control 3	PRS-1464-0.1mg
Concentration	1 mg/ml
Buffer	XBP-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Application Note	WB: 0.25-2 μg/mL; IF: 20 μg/mL; ICC: 10 μg/mL; IHC: 2-5 μg/mL. Antibody validated: Western Blot in human samples; Immunofluorescence in human and rat samples; Immunocytochemistry in human samples; Immunohistochemistry in human, mouse, and rat samples. All other applications and species not yet tested.
Homology	Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen	Anti-XBP-1 antibody (3687) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human XBP-1. The immunogen is located within amino acids 40 - 90 of XBP-1.
Clonality	Polyclonal
NCBI Official Name	X-box binding protein 1
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Validation	KO Validation (Figure 2): XBP-1 expression detected by anti-XBP-1 antibodies was observed in wild type HeLa cells, but not in the XBP-1 knockout HeLa cells. Recombinant Protein Test (Figure 3): Anti-XBP-1 antibodies (3687) detected human XBP-1 recombinant protein at different concentrations.

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Menge: 0.02 mg

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Lieferung vsl. bis 31.07.2025

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