Bit1 Antibody

Apoptosis

Bit1 antibody was raised against a 16 amino acid synthetic peptide from near the center of human Bit1.

The immunogen is located within amino acids 80 - 130 of Bit1.

Cat. No. 1224 - Daudi Cell Lysate

Cat. No. 11-801 - Human Small Intestine Tissue Slide

Polyclonal

Unconjugated

1 mg/mL

Cat. No. 3605P – Bit1 Peptide

None

NP_057161

51651

PTRH2

Homo sapiens

Bit1 Antibody: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process. Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE. Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.

Jan Y, Matter M, Pai J-t, et al. A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and groucho/TLE corepressors. Cell 2004; 116:751-762.

Western blot analysis of Bit1 in Daudi cell lysate with Bit1 antibody at (A) 1, (B) 2, and (C) 4 μ g/mL.

Immunofluorescence of Bit1 in Human Small Intestine cells with Bit1 antibody at 20 μ g/mL.