RPS6K1 Antibody

blue ice or RT

Predicted species reactivity based on immunogen sequence: Rat: (100%), Chicken: (100%), Mouse: (100%)

RPS6K1antibody can be used for the detection of RPS6K1 by Western blot at 2.5 - 10 μ g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ g/mL. For immunofluorescence start at 20 μ g/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Cat. No. 17-005 - Jurkat Cell Slide

Polyclonal

Unconjugated

1 mg/mL

Cat. No. 3511P – RPS6K1 Peptide

None

AAC82497

6195

RPS6KA1

Homo sapiens

RPS6K1 Antibody: Ribosomal protein S6 kinase 1 (RPS6K1) is the best characterized effector of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Nutrients and growth factors stimulate a complex including TOR, raptor (regulatory associated protein of TOR), and GbetaL to phosphorylate RPS6K1 and the eukaryotic initiation factor 4E binding protein (4EBP1), leading to increased protein synthesis and cell growth. RPS6K1 is thought to desensitize tissues to insulin as mice deficient in RPS6K1 have been shown to be hypersensitive to insulin and impervious to obesity-induced insulin resistance seen in wild type obese mice.

Inoki K, Ouyang H, Li Y, et al. Signaling by target of rapamycin proteins in cell growth control. Microbiol. Mol. Biol. Rev. 2005; 69:79-100.

Um SH, Frigerio F, Watanabe M, et al. Absences of S6K1 protects against age- and diet-induced obesity while enhancing insulin sensitivity. Nature 2004; 431:200-5.

Immunocytochemistry of RPS6K1 in Jurkat cells with RPS6K1 antibody at 10 μ g/mL.