ArtNr |
PRS-34-149-100ug |
Hersteller |
ProSci
|
Menge |
100 ug |
Kategorie |
|
Typ |
Antibody |
Format |
Liquid |
Applikationen |
FC, IF, IHC |
Specific against |
Human, Mouse, Monkey |
Host |
Mouse |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
FOXP3 Antibody: JM2, AIID, IPEX, PIDX, XPID, DIETER, JM2, Forkhead box protein P3, Scurfin |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Innate Immunity |
Immunogen |
Full-length His-tagged mouse FOXP3 protein was used as the immunogen for this FOXP3 antibody. |
Applications |
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 0.5-1 ug/ml Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2) The optimal dilution of the FOXP3 antibody for each application should be determined by the researcher. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Purification |
Protein G affinity chromatography |
Clonality |
Monoclonal |
Clone |
SPM579 |
Isotype |
IgG1, kappa |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
Concentration |
0.2 mg/mL |
Storage Conditions |
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
FOXP3 |
Ncbi Gene Id # |
50943 |
User Note |
Optimal dilutions for each application to be determined by the researcher |
Ncbi Official Symbol |
FOXP3 |
Ncbi Official Full Name |
forkhead box P3 |
Ncbi Organism |
homo sapiens |
Swissprot # |
Q9BZS1 |
Background |
Recognizes a protein of 47-55kDa, which is identified as FOXP3. Its precise epitope is not known, but it has been mapped to the N-terminal portion of the protein. The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. During early development, FOXP1 and FOXP2 are expressed abundantly in the lung, with lower levels of expression in neural, intestinal and cardiovascular tissues, where they act as transcription repressors. FOXP1 is widely expressed in adult tissues, while neoplastic cells often exhibit a dramatic change in expression level or localization of FOXP1. Mutations in FOXP3 gene cause IPEX, a fatal, X-linked inherited disorder characterized by immune dysregulation. The FOXP3 protein is essential for normal immune homeostasis. Specifically, FOXP3 represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation. |
1st Image Caption |
Formalin-fixed, paraffin-embedded human testicular carcinoma stained with FOXP3 antibody (SPM579). |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.