ArtNr |
PRS-34-146-100ug |
Hersteller |
ProSci
|
Menge |
100 ug |
Kategorie |
|
Typ |
Antibody Monoclonal |
Format |
Liquid |
Applikationen |
FC, IHC |
Specific against |
Human, Mouse, Rat, Chicken, Pig |
Host |
Mouse |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
NEFH, 200 kDa neurofilament protein, KIAA0845, NFH, NF-H |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Obesity |
Immunogen |
The Triton-X 100 insoluble protein fraction from rat brain was used as the immunogen for this anti-NF-H antibody. |
Applications |
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) The optimal dilution of the anti-NF-H antibody for each application should be determined by the researcher. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Purification |
Protein G affinity chromatography |
Clonality |
Monoclonal |
Clone |
SPM203 |
Isotype |
IgG1 |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
Concentration |
0.2 mg/mL |
Storage Conditions |
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
NEFH |
Ncbi Gene Id # |
4744 |
User Note |
Optimal dilutions for each application to be determined by the researcher |
Ncbi Official Symbol |
NEFH |
Ncbi Official Full Name |
neurofilament, heavy polypeptide |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P12036 |
Background |
This mAb reacts with a 200kDa protein, identified as heavy subunit of neurofilaments. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament. |
1st Image Caption |
Formalin-fixed, paraffin-embedded human cerebellum stained with anti-NF-H antibody (SPM203). |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.