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SOX10 Antibody [SBX10-1]

ArtNr PRS-33-852-100ug
Hersteller ProSci
Menge 100 ug
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen WB, IF, IHC-P
Specific against Human
Host Mouse
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias SOX10 Antibody: SRY (sex determining region Y)-box 10, DOM,WS4, PCWH, WS2E, WS4C
Lieferbar
Shipping
blue ice or RT
By Research Area
Signal Transduction, Stem Cell
Immunogen
Amino acids 115-269 of the human protein were used as the immunogen for the SOX10 antibody.
Applications
Immunofluorescence: 1-2 ug/ml

Western blot: 1-2 ug/ml

Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT

Prediluted IHC only format: incubate for 30 min at RT (1)

Titering of the SOX10 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification
Protein G affinity chromatography
Clonality
Monoclonal
Clone
SBX10-1
Isotype
IgG2b, kappa
Conjugate
Unconjugated
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration
0.2 mg/mL
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
SOX10
Ncbi Gene Id #
6663
User Note
Optimal dilutions for each application to be determined by the researcher
Ncbi Official Symbol
SOX10
Ncbi Official Full Name
SRY (sex determining region Y)-box 10
Ncbi Organism
Homo sapiens
Swissprot #
P56693
Background
SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. It may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. [RefSeq]
1st Image Caption
Analysis of HuProt(TM) microarray containing more than 19, 000 full-length human proteins using SOX10 antibody (clone SBX10-1). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
2nd Image Caption
Western blot testing of 1) partial recombinant protein and 2) A375 cell lysate using SOX10 antibody. Predicted molecular weight ~50 kDa.
3rd Image Caption
IHC testing of FFPE melanoma with SOX10 antibody (clone SBX10-1). Required HIER: boil sections in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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