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MCM7 Antibody [MCM7/1468]

ArtNr PRS-33-847-100ug
Hersteller ProSci
Menge 100 ug
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen WB, FC, IF, IHC-P
Specific against Human
Host Mouse
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias DNA replication licensing factor MCM7, CDC47 homolog, P11-MCM3, MCM7, CDC47, MCM2
Lieferbar
Shipping
blue ice or RT
By Research Area
Other
Immunogen
A human partial recombinant protein corresponding to amino acids 195-319 was used as the immunogen for the MCM7 antibody.
Applications
Immunofluorescence: 0.5-1 ug/ml

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT

Prediluted IHC only format: incubate for 30 min at RT (1)

Titering of the MCM7 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification
Protein G affinity chromatography
Clonality
Monoclonal
Clone
MCM7/1468
Isotype
IgG2b
Conjugate
Unconjugated
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration
0.2 mg/mL
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
MCM7
Ncbi Gene Id #
4176
User Note
Optimal dilutions for each application to be determined by the researcher
Ncbi Official Symbol
MCM7
Ncbi Official Full Name
minichromosome maintenance complex component 7
Ncbi Organism
Homo sapiens
Swissprot #
P33993
Background
MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB.
1st Image Caption
Analysis of HuProt(TM) microarray containing more than 19, 000 full-length human proteins using MCM7 antibody (clone MCM7/1468). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
2nd Image Caption
IHC testing of FFPE human tonsil with MCM7 antibody (clone MCM7/1468). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
3rd Image Caption
SDS-PAGE Analysis of Purified, BSA-Free MCM7 Antibody (clone MCM7/1468). Confirmation of Integrity and Purity of the Antibody.

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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