ArtNr |
PRS-33-842-100ug |
Hersteller |
ProSci
|
Menge |
100 ug |
Kategorie |
|
Typ |
Antibody Monoclonal |
Format |
Liquid |
Applikationen |
WB, FC, IF, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Mouse |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
ENO2, Enolase 2, Enolase 2 (gamma, neuronal), Neurone-specific enolase, Gamma-enolase, Neural enolase, Neuron specific gamma enolase, Neuron-specific enolase, NSE |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Obesity, Neuroscience, Stem Cell |
Immunogen |
Amino acids 416-433 of human NSE were used as the immunogen for this Neuron Specific Enolase antibody. |
Applications |
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 1-2 ug/ml Immunohistochemistry (FFPE): 0.1-0.2 ug/ml for 30 min at RT Prediluted IHC only format: incubate for 30 min at RT (1) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Neuron Specific Enolase antibody to be titered up or down for optimal performance. 1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Purification |
Protein G affinity chromatography |
Clonality |
Monoclonal |
Clone |
ENO2/1462 |
Isotype |
IgG2b |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
Concentration |
0.2 mg/mL |
Storage Conditions |
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
ENO2 |
Ncbi Gene Id # |
2026 |
User Note |
Optimal dilutions for each application to be determined by the researcher |
Ncbi Official Symbol |
ENO2 |
Ncbi Official Full Name |
enolase 2 (gamma, neuronal) |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P09104 |
Background |
This mAb recognizes a protein of about 50 kDa which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament. |
1st Image Caption |
Analysis of HuProt(TM) microarray containing more than 19, 000 full-length human proteins using Neuron Specific Enolase antibody (clone ENO2/1462). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target. |
2nd Image Caption |
IHC testing of FFPE human pheochromocytoma with NSE antibody (clone ENO2/1462). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min. |
3rd Image Caption |
IHC testing of FFPE mouse pancreas with NSE antibody (clone ENO2/1462). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min. |
4th Image Caption |
IHC testing of FFPE rat heart with NSE antibody (clone ENO2/1462). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min. |
5th Image Caption |
SDS-PAGE Analysis of Purified, BSA-Free Neuron Specific Enolase Antibody (clone ENO2/1462). Confirmation of Integrity and Purity of the Antibody. |
Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.
Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.