ArtNr |
PRS-33-794-100ug |
Hersteller |
ProSci
|
Menge |
100 ug |
Kategorie |
|
Typ |
Antibody Monoclonal |
Format |
Liquid |
Applikationen |
WB, FC, IF, ELISA, IHC-P |
Specific against |
Human |
Host |
Mouse |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
Chymotrypsin-like elastase family member 3B, Elastase IIIB, Elastase-3B, Protease E, CELA3B, ELA3B |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cell Cycle, Signal Transduction |
Immunogen |
A recombinant partial protein (aa 82-238) was used as the immunogen for the CELA3B antibody. |
Applications |
Western blot: 0.5-1 ug/ml
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
IF: 1-2 ug/ml
ELISA: 2-4 ug/ml; order BSA free formulation for coating
IHC (FFPE): 1-2 ug/ml for 30 min at RT
Optimal dilution of the CELA3B antibody should be determined by the researcher. |
Purification |
Protein G affinity chromatography |
Clonality |
Monoclonal |
Clone |
CELA3B/1757 |
Isotype |
IgG1, kappa |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
Concentration |
0.2 mg/mL |
Storage Conditions |
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
CELA3B |
Ncbi Gene Id # |
23436 |
User Note |
Optimal dilutions for each application to be determined by the researcher |
Ncbi Official Symbol |
CELA3B |
Ncbi Official Full Name |
Chymotrypsin-like elastase family member 3B |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P08861 |
Background |
This mAb recognizes a protein of ~30kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B), or Elastase 3B. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays. |
1st Image Caption |
IHC testing of human pancreas with CELA3B antibody (clone CELA3B/1757). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min. |
2nd Image Caption |
SDS-PAGE Analysis of Purified, BSA-Free CELA3B Antibody (clone CELA3B/1757). Confirmation of Integrity and Purity of the Antibody. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.