ArtNr |
PRS-33-554-100ug |
Hersteller |
ProSci
|
Menge |
100 ug |
Kategorie |
|
Typ |
Antibody Monoclonal |
Format |
Liquid |
Applikationen |
WB, FC, IF, IHC |
Specific against |
Human |
Host |
Mouse |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Immunology |
Immunogen |
Purified human Ig Gamma Chain was used as the immunogen for this IgG antibody. |
Applications |
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 0.5-1 ug/ml Immunohistochemistry (Formalin/paraffin) 0.5-1 ug/ml for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The optimal dilution of the IgG antibody for each application should be determined by the researcher. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Purification |
Protein G affinity chromatography |
Clonality |
Monoclonal |
Clone |
IG217 |
Isotype |
IgG1, kappa |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
Concentration |
0.2 mg/mL |
Storage Conditions |
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
IGHG1, IGHG2, IGHG3, IGHG4 |
Ncbi Gene Id # |
3500, 3501, 3502, 3503 |
User Note |
Optimal dilutions for each application to be determined by the researcher |
Ncbi Official Symbol |
IGHG1, IGHG2, IGHG3, IGHG4 |
Ncbi Official Full Name |
immunoglobulin heavy constant gamma 1 (G1m marker), immunoglobulin heavy constant gamma 2 (G2m marker), immunoglobulin heavy constant gamma 3 (G3m marker), immunoglobulin heavy constant gamma 4 (G4m marker) |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P01857, P01859, P01860, P01861 |
Background |
Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. |
1st Image Caption |
Formalin-fixed, paraffin-embedded human tonsil stained with IgG antibody (IG217) |
2nd Image Caption |
SDS-PAGE Analysis of Purified, BSA-Free IgG Antibody (clone IG217). Confirmation of Integrity and Purity of the Antibody. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.