ArtNr |
PRS-33-394-100ug |
Hersteller |
ProSci
|
Menge |
100 ug |
Kategorie |
|
Typ |
Antibody Monoclonal |
Format |
Liquid |
Applikationen |
WB, FC, IF |
Specific against |
Human, Monkey, Canine |
Host |
Mouse |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Immunology |
Immunogen |
Activated human peripheral blood mononuclear cells were used as the immunogen for the HLA-DRB1 antibody. |
Applications |
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Optimal dilution of the HLA-DRB1 antibody should be determined by the researcher. |
Purification |
Protein G affinity chromatography |
Clonality |
Monoclonal |
Clone |
L243 |
Isotype |
IgG2a, kappa |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
Concentration |
0.2 mg/mL |
Storage Conditions |
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
HLA-DRB1 |
Ncbi Gene Id # |
3123 |
User Note |
Optimal dilutions for each application to be determined by the researcher |
Ncbi Official Symbol |
HLA-DRB1 |
Ncbi Official Full Name |
major histocompatibility complex, class II, DR beta 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P01911 |
Background |
This mAb reacts with the HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. It binds a conformational epitope on HLA-DR, which depends on the correct folding of the alpha/beta heterodimer. This mAb has been reported to block mixed lymphocyte reactions. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kD alpha (heavy) chain and a 28kD beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. |
1st Image Caption |
SDS-PAGE Analysis of Purified, BSA-Free HLA-DRB1 Antibody (clone L243). Confirmation of Integrity and Purity of the Antibody. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.