ArtNr |
PRS-33-285-100ug |
Hersteller |
ProSci
|
Menge |
100 ug |
Kategorie |
|
Typ |
Antibody Monoclonal |
Format |
Liquid |
Applikationen |
FC, IF, IHC |
Specific against |
Human |
Host |
Mouse |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20, MS4A1, CD20 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Immunology, Signal Transduction |
Immunogen |
Recombinant human MS4A1/CD20 protein was used as the immunogen for this anti-CD20 antibody. |
Applications |
Flow Cytometry: 0.5-1 ug/million cells IF: 0.5-1 ug/ml Immunohistology (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-CD20 antibody to be titered up or down for optimal performance. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Purification |
Protein G affinity chromatography |
Clonality |
Monoclonal |
Clone |
SPM618 |
Isotype |
IgG2a, kappa |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
Concentration |
0.2 mg/mL |
Storage Conditions |
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
MS4A1 |
Ncbi Gene Id # |
931 |
User Note |
Optimal dilutions for each application to be determined by the researcher |
Ncbi Official Symbol |
MS4A1 |
Ncbi Official Full Name |
membrane-spanning 4-domains, subfamily A, member 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P11836 |
Background |
This antibody recognizes a protein of 33-37kDa, identified as CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. It is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B-cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression. |
1st Image Caption |
IHC testing of anti-CD20 antibody and FFPE human tonsil tissue |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.