ArtNr |
PRS-32-102-0.1mg |
Hersteller |
ProSci
|
Menge |
0.1 mg |
Kategorie |
|
Typ |
Antibody Primary |
Applikationen |
WB, ELISA |
Specific against |
Human |
Host |
Mouse |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Phosphorylated heat- and acid-stable protein regulated by insulin 1, 4E-BP1, BP-1, 4EBP1, PHAS-I |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Signal Transduction |
Immunogen |
Ni-NTA purified truncated recombinant 4E-BP1 expressed in E. Coli strain BL21 (DE3). |
Applications |
Western Blot:1:500 - 1:1, 000 IHC(P):1:500 - 1:1, 000 ELISA:Propose dilution 1:10, 000. Determining optimal working dilutions by titration test. |
Clonality |
Monoclonal |
Clone |
4B6G10 |
Isotype |
IgG1 |
Conjugate |
Unconjugated |
Buffer |
Ascitic fluid containing 0.03% sodium azide. |
Storage Conditions |
4E-BP1 monoclonal antibody can be stored at -20˚ C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
EIF4EBP1 |
Accession # |
Q13541 |
Protein Gi # |
34921508 |
Ncbi Gene Id # |
1978 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
EIF4EBP1 |
Ncbi Official Full Name |
eukaryotic translation initiation factor 4E binding protein 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q13541 |
Background |
4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1), also called ELF4EBP1/BP-1/PHAS-I , which is located on chromosome 8p12, with 118-amino acid protein (about 13 kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24 kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction. |
Background References 1 |
Pause, A. et al. 1994.Nature. 371:762–767. |
Background References 2 |
Fadden, P. et al. 1997. J. Biol. Chem. 272:10240–10247. |
1st Image Caption |
Western blot analysis using 4E - BP1 monoclonal antibody against truncated 4E - BP1 recombinant protein and A431 cell lysate. |
2nd Image Caption |
Immunohistochemical analysis of paraffin - embedded human pancreas carcinoma showing cytoplasmic and membrane location using 4E - BP1 antibody with DAB staining. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.