ArtNr |
PRS-3023-0.1mg |
Hersteller |
ProSci
|
Menge |
0.1 mg |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Bcl-rambo Antibody: MIL1, BCL-RAMBO, Bcl2-L-13, MIL1, CD003, Bcl-2-like protein 13, Bcl-rambo |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Apoptosis |
Immunogen |
Bcl-rambo antibody was raised against a 15 amino acid synthetic peptide from near the center of human Bcl-rambo.
The immunogen is located within amino acids 280 - 330 of Bcl-rambo. |
Applications |
Bcl-rambo antibody can be used for the detection of Bcl-rambo by Western blot at 2 and 4 μ g/mL. Antibody can also be used for immunohistochemistry starting at 10 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Positive Control 1 |
Cat. No. 1204 - K562 Cell Lysate |
Positive Control 2 |
Cat. No. 1301 - Human Heart Tissue Lysate |
Positive Control 3 |
Cat. No. 10-501 - Human Heart Tissue Slide |
Purification |
Bcl-rambo Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
Bcl-rambo Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
Bcl-rambo antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 3023P – Bcl-rambo Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
BCL2L13 |
Accession # |
AAH07658 |
Protein Gi # |
14043326 |
Ncbi Gene Id # |
23786 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
BCL2L13 |
Ncbi Official Full Name |
BCL2-like 13 (apoptosis facilitator) |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9BXK5 |
Background |
Bcl-rambo Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Members of the Bcl-2 family are known to be critical regulators of this process. These proteins are characterized by the presence of several conserved motifs termed Bcl-2 homology (BH) domains. A novel, widely expressed member termed Bcl-rambo was recently identified. This protein is localized to mitochondria in mammalian cells and its overexpression induces apoptosis which could be blocked by co-expression of inhibitor of apoptosis proteins (IAPs) such as XIAP, cIAP1, and cIAP2. Bcl-rambo shows overall homology to the anti-apoptotic members containing BH motifs, but unlike Bcl-2, the C-terminal membrane anchor of Bcl-rambo is preceded by a unique 250 amino acid insertion. This region by itself can induce apoptosis more efficiently than the Bcl-2 homology regions, suggesting that Bcl-rambo may be important other pro-apoptotic pathways. |
Background References 1 |
Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7. |
Background References 2 |
Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607. |
Background References 3 |
Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35. |
Background References 4 |
Kataoka T, Holler N, Michau O, et al. Bcl-rambo, a novel Bcl-2 homologue that induces apoptosis via its unique C-terminal extension. J. Biol. Chem. 2001; 276:19548-54., |
1st Image Caption |
Western blot analysis of Bcl-rambo in K562 cell lysate with Bcl-rambo antibody at (A) 2 and (B) 4 μ g/mL. |
2nd Image Caption |
Immunohistochemistry of Bcl-rambo in human heart tissue with Bcl-rambo antibody at 10 μ g/mL. |
3rd Image Caption |
Immunofluorescence of Bcl-rambo in Human Heart cells with Bcl-rambo antibody at 20 μ g/mL. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.