ArtNr |
PRS-29-851-100ul |
Hersteller |
ProSci
|
Menge |
100 ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
RAD18, RNF73 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer , Apoptosis , Homeostasis |
Immunogen |
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RAD18. |
Applications |
RAD18 antibody can be used for detection of RAD18 by ELISA at 1:312500. RAD18 antibody can be used for detection of RAD18 by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000. |
Positive Control 1 |
721_B Cell Lysate |
Predicted Molecular Weight |
56 kDa |
Purification |
Antibody is purified by peptide affinity chromatography method. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Concentration |
batch dependent |
Storage Conditions |
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store RAD18 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
RAD18 |
Accession # |
NP_064550 |
Protein Gi # |
256818821 |
Ncbi Gene Id # |
56852 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
RAD18 |
Ncbi Official Full Name |
RAD18 homolog (S. cerevisiae) |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9NS91 |
Background |
RAD18 is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, RAD18 is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
Background References 1 |
Tsuji, Y., (2008) Genes Cells 13 (4), 343-354. |
1st Image Caption |
Antibody used in WB on Human 721_B at 0.2-1 ug/ml. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.