CARS Antibody

RNA Binding , Cancer

CARS antibody can be used for detection of CARS by ELISA at 1:62500. CARS antibody can be used for detection of CARS by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.

81 kDa, 95 kDa, 85 kDa, 83 kDa

Polyclonal

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store CARS antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

None

NP_001014438

833

CARS

Homo sapiens

CARS is a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.