EGR4 Antibody

Transcription

EGR4 antibody can be used for detection of EGR4 by ELISA at 1:312500. EGR4 antibody can be used for detection of EGR4 by western blot at 0.0625 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.

51 kDa

Polyclonal

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store EGR4 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

None

NP_001956

1961

EGR4

Homo sapiens

The nerve growth factor-induced clone C (NGFI-C/EGR4) gene is a zinc-finger transcription factor that is rapidly induced by nerve growth factor in rat pheochromocytoma PC12 cells and by seizure in brain. NGFI-C/EGR4 is closely related to the previously described early response genes, nerve growth factor-induced clone A (NGFI-A or EGR1), EGR2, and EGR3. These four early response (immediate early) proteins all contain very similar zinc-finger DNA binding domains and five highly homologous subdomains. EGR-4 functionally cooperate with NFAT proteins and induce expression of IL-2 and TNFalpha. Early growth response proteins (EGR) and nuclear factors of activated T cells (NFAT) form heterodimers and regulate proinflammatory cytokine gene expression.

Antibody used in WB on Human Jurkat 0.0625 ug/ml.