ArtNr |
PRS-25-766-100ul |
Hersteller |
ProSci
|
Menge |
100 ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
EIF2AK1, HRI, KIAA1369, HCR |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Signal Transduction |
Immunogen |
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF2AK1. |
Applications |
EIF2AK1 antibody can be used for detection of EIF2AK1 by ELISA at 1:62500. EIF2AK1 antibody can be used for detection of EIF2AK1 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000. |
Positive Control 1 |
Cat. No. XBL-10410 - Fetal Lung Tissue Lysate |
Predicted Molecular Weight |
71 kDa |
Purification |
Antibody is purified by peptide affinity chromatography method. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Concentration |
batch dependent |
Storage Conditions |
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store EIF2AK1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
EIF2AK1 |
Accession # |
NP_055228 |
Protein Gi # |
11125768 |
Ncbi Gene Id # |
27102 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
EIF2AK1 |
Ncbi Official Full Name |
eukaryotic translation initiation factor 2-alpha kinase 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9BQI3 |
Background |
EIF2AK1 acts at the level of translation initiation to downregulate protein synthesis in response to stress. The protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene.The HRI gene is localized to 7p22 where its 3' end slightly overlaps the 3' end of the gene JTV1. The two genes are transcribed from opposite strands. Studies in rat and rabbit suggest that the HRI gene product phosphorylates the alpha subunit of eukaryotic initiation factor 2. Its kinase activity is induced by low levels of heme and inhibited by the presence of heme. Sequence Note: The sequence AF181071.1 is a chimeric mRNA clone. Only the heme-regulated initiation factor 2-alpha kinase region was propagated into this RefSeq record. |
Background References 1 |
Olsen, J.V., (2006) Cell 127 (3), 635-648. |
1st Image Caption |
Antibody used in WB on Human Lung at 0.2-1 ug/ml. |
2nd Image Caption |
Antibody used in WB on Human Hela at 1 ug/ml. |
3rd Image Caption |
Antibody used in WB on Human HEK293T cells at 1 ug/ml. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.