SP3 Antibody

Transcription , Cancer

SP3 antibody can be used for detection of SP3 by ELISA at 1:312500. SP3 antibody can be used for detection of SP3 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.

82 kDa

Polyclonal

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SP3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

None

NP_003102

6670

SP3

Homo sapiens

SP3 is a transcriptional factor that can act as an activator or repressor, probably in a isoform-specific manner. SP3 binds to GT and GC boxes promoters elements.This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted.

Antibody used in IHC on Human Prostate at 5.0 ug/ml.

Antibody used in WB on Human U2OS at 1:1000.