Vergleich

YWHAH Antibody

ArtNr PRS-23-787-50uL
Hersteller ProSci
Menge 50 uL
Kategorie
Typ Antibody Primary
Format Liquid
Applikationen WB, IF, IHC
Specific against Human, Mouse, Rat
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias YWHAH, YWHA1
Lieferbar
Shipping
blue ice or RT
By Research Area
Apoptosis, Cell Cycle, Signal Transduction, Stem Cell
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-246 of human YWHAH (NP_003396.1).
Applications
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Positive Control 1
U-87MG
Positive Control 2
LO2
Positive Control 3
Raji
Positive Control 4
Jurkat
Positive Control 5
Mouse heart
Positive Control 6
Mouse kidney
Predicted Molecular Weight
Observed: 28kDa
Purification
Affinity purification
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
YWHAH
Ncbi Gene Id #
7533
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
YWHAH
Ncbi Official Full Name
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
Ncbi Organism
Homo sapiens
Swissprot #
Q04917
Background
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder.
1st Image Caption
Western blot analysis of extracts of various cell lines, using YWHAH antibody (23-787) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
2nd Image Caption
Immunofluorescence analysis of NIH-3T3 cells using YWHAH Polyclonal Antibody (23-787) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
3rd Image Caption
Immunofluorescence analysis of NIH-3T3 cells using YWHAH Polyclonal Antibody (23-787) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 uL
Lieferbar: In stock
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