ArtNr |
PRS-23-621-50uL |
Hersteller |
ProSci
|
Menge |
50 uL |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
REV1L, REV1-like (yeast), REV1, REV1 protein, REV1 (yeast homolog)- like, REV1L |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Other |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 120-345 of human REV1 (NP_057400.1). |
Applications |
WB: 1:500 - 1:2000 |
Positive Control 1 |
MCF7 |
Predicted Molecular Weight |
Observed: 145kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
REV1 |
Ncbi Gene Id # |
51455 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
REV1 |
Ncbi Official Full Name |
REV1, polymerase (DNA directed) |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9UBZ9 |
Background |
This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. |
1st Image Caption |
Western blot analysis of extracts of MCF-7 cells, using REV1 antibody (23-621) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.