« Zurück
Home /
MRPL12 Antibody

MRPL12 Antibody

ArtNr	PRS-23-507-50uL
Hersteller	ProSci
Menge	50 uL
Kategorie	Antibodies Immunohistochemistry Western Blot Primary Antibodies
Typ	Antibody Primary
Format	Liquid
Applikationen	WB, IHC
Specific against	Human
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	5c5-2, L12mt, MRP-L31/34, MRPL7, MRPL7/L12, RPML12, 39S ribosomal protein L12, mitochondrial, MRP-L12, mitochondrial large ribosomal subunit protein bL12m
Lieferbar
Shipping	blue ice or RT
By Research Area	Other
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human MRPL12 (NP_002940.2).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:100
Positive Control 1	A-431
Positive Control 2	22Rv1
Positive Control 3	A-549
Predicted Molecular Weight	Observed: 21kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	MRPL12
Ncbi Gene Id #	6182
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	MRPL12
Ncbi Official Full Name	mitochondrial ribosomal protein L12
Ncbi Organism	Homo sapiens
Swissprot #	P52815
Background	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex.
1st Image Caption	Western blot analysis of extracts of various cell lines, using MRPL12 antibody (23-507) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
2nd Image Caption	Immunohistochemistry of paraffin-embedded human esophageal smooth muscle using MRPL12 antibody (23-507) at dilution of 1:100 (40x lens).

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Arbeitsanleitung (Data Sheet) anfordern

Produkdatenblatt anfordern

Sicherheitsdatenblatt anfordern

Einfache Bestellung Periodische Bestellung

Menge: 50 uL

lieferbar

Lieferung vsl. bis 06.09.2024

Express-Lieferung: 11 Tage sparen

Vergleichen

Auf den Wunschzettel

Weiterempfehlen per Mail