ArtNr |
PRS-23-297-50uL |
Hersteller |
ProSci
|
Menge |
50 uL |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, IHC |
Specific against |
Human, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
apolipoprotein L1, apolipoprotein L-I, OTTHUMP00000197987, OTTHUMP00000197986, OTTHUMP00000197985, OTTHUMP00000197984, APOL-I, APOL, APO-L, apolipoprotein L, 1, APOL1 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 179-398 of human APOL1 (NP_003652.2). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100 |
Positive Control 1 |
Raji |
Positive Control 2 |
Jurkat |
Positive Control 3 |
HepG2 |
Predicted Molecular Weight |
Observed: 38kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
APOL1 |
Ncbi Gene Id # |
8542 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
APOL1 |
Ncbi Official Full Name |
apolipoprotein L, 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
O14791 |
Background |
This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using APOL1 antibody (23-297) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s. |
2nd Image Caption |
Immunohistochemistry of paraffin-embedded rat brain using APOL1 antibody (23-297) at dilution of 1:100 (40x lens). |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.