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ADRA1A Antibody

ArtNr PRS-23-244-50uL
Hersteller ProSci
Menge 50 uL
Kategorie
Typ Antibody Primary
Format Liquid
Applikationen WB, IF, IHC
Specific against Human, Mouse, Rat
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias ADRA1A, adrenergic, alpha-1A-, receptor, ADRA1C, ADRA1L1, ALPHA1AAR, G protein coupled receptor, OTTHUMP00000125310, OTTHUMP00000125311, OTTHUMP00000125312, OTTHUMP00000125313, adrenergic, alpha-1A-, receptor variant 1, adrenergic, alpha-1A-, receptor var
Lieferbar
Shipping
blue ice or RT
By Research Area
Apoptosis, Cell Cycle, Neuroscience, Signal Transduction
Immunogen
A synthetic peptide of human ADRA1A
Applications
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:200
Positive Control 1
BT-474
Positive Control 2
HL-60
Positive Control 3
22Rv1
Positive Control 4
Raji
Positive Control 5
Mouse kidney
Positive Control 6
Mouse heart
Predicted Molecular Weight
Observed: 60kDa
Purification
Affinity purification
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
ADRA1A
Ncbi Gene Id #
148
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
ADRA1A
Ncbi Official Full Name
adrenoceptor alpha 1A
Ncbi Organism
Homo sapiens
Swissprot #
P35348
Background
Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties.
1st Image Caption
Western blot analysis of extracts of various cell lines, using ADRA1A antibody (23-244) at 1:500 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
2nd Image Caption
Immunohistochemistry of paraffin-embedded rat lung using ADRA1A antibody (23-244) at dilution of 1:100 (40x lens).
3rd Image Caption
Immunohistochemistry of paraffin-embedded rat heart using ADRA1A antibody (23-244) at dilution of 1:100 (40x lens).
4th Image Caption
Immunohistochemistry of paraffin-embedded mouse heart using ADRA1A antibody (23-244) at dilution of 1:100 (40x lens).
5th Image Caption
Immunofluorescence analysis of HeLa cells using ADRA1A antibody (23-244) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 uL
Lieferbar: In stock
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