ArtNr |
PRS-23-244-50uL |
Hersteller |
ProSci
|
Menge |
50 uL |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, IF, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
ADRA1A, adrenergic, alpha-1A-, receptor, ADRA1C, ADRA1L1, ALPHA1AAR, G protein coupled receptor, OTTHUMP00000125310, OTTHUMP00000125311, OTTHUMP00000125312, OTTHUMP00000125313, adrenergic, alpha-1A-, receptor variant 1, adrenergic, alpha-1A-, receptor var |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Apoptosis, Cell Cycle, Neuroscience, Signal Transduction |
Immunogen |
A synthetic peptide of human ADRA1A |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:200 |
Positive Control 1 |
BT-474 |
Positive Control 2 |
HL-60 |
Positive Control 3 |
22Rv1 |
Positive Control 4 |
Raji |
Positive Control 5 |
Mouse kidney |
Positive Control 6 |
Mouse heart |
Predicted Molecular Weight |
Observed: 60kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
ADRA1A |
Ncbi Gene Id # |
148 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
ADRA1A |
Ncbi Official Full Name |
adrenoceptor alpha 1A |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P35348 |
Background |
Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using ADRA1A antibody (23-244) at 1:500 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s. |
2nd Image Caption |
Immunohistochemistry of paraffin-embedded rat lung using ADRA1A antibody (23-244) at dilution of 1:100 (40x lens). |
3rd Image Caption |
Immunohistochemistry of paraffin-embedded rat heart using ADRA1A antibody (23-244) at dilution of 1:100 (40x lens). |
4th Image Caption |
Immunohistochemistry of paraffin-embedded mouse heart using ADRA1A antibody (23-244) at dilution of 1:100 (40x lens). |
5th Image Caption |
Immunofluorescence analysis of HeLa cells using ADRA1A antibody (23-244) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.