ArtNr |
PRS-23-035-50uL |
Hersteller |
ProSci
|
Menge |
50 uL |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, IF |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
3PK, MAPKAP-K3, MAPKAP3, MAPKAPK-3, MDPT3, MK-3, MAP kinase-activated protein kinase 3, MAPKAP kinase 3, chromosome 3p kinase, mitogen-activated protein kinase-activated protein kinase 3 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cell Cycle, Immunology, Signal Transduction |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human MAPKAPK3 (NP_004626.1). |
Applications |
WB: 1:500 - 1:2000
IF: 1:50 - 1:200 |
Positive Control 1 |
HeLa |
Positive Control 2 |
Jurkat |
Positive Control 3 |
MCF7 |
Positive Control 4 |
Mouse lung |
Positive Control 5 |
Mouse heart |
Predicted Molecular Weight |
Observed: 38kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
MAPKAPK3 |
Ncbi Gene Id # |
7867 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
MAPKAPK3 |
Ncbi Official Full Name |
MAPK activated protein kinase 3 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q16644 |
Background |
This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. Alternate splicing results in multiple transcript variants that encode the same protein. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using MAPKAPK3 antibody (23-035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s. |
2nd Image Caption |
Immunofluorescence analysis of A549 cells using MAPKAPK3 antibody (23-035). Blue: DAPI for nuclear staining. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.