ArtNr |
PRS-22-922-50uL |
Hersteller |
ProSci
|
Menge |
50 uL |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, IF, IP, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
RIPK1 Antibody: RIP, RIP1, RIP, Cell death protein RIP, RIP-1 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Apoptosis, Cancer, Cell Cycle, Immunology, Signal Transduction |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 170-440 of human RIPK1 (NP_003795.2). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:200 |
Positive Control 1 |
Jurkat |
Positive Control 2 |
HT-1080 |
Positive Control 3 |
DU145 |
Positive Control 4 |
K-562 |
Positive Control 5 |
NIH/3T3 |
Positive Control 6 |
Mouse liver |
Predicted Molecular Weight |
Observed: 76kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
RIPK1 |
Ncbi Gene Id # |
8737 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
RIPK1 |
Ncbi Official Full Name |
receptor (TNFRSF)-interacting serine-threonine kinase 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q13546 |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using RIPK1 antibody (22-922) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
2nd Image Caption |
Western blot analysis of extracts of various cell lines, using RIPK1 antibody (22-922) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. |
3rd Image Caption |
Immunohistochemistry of paraffin-embedded human tonsil using RIPK1 antibody (22-922) at dilution of 1:100 (40x lens). |
4th Image Caption |
Immunohistochemistry of paraffin-embedded rat lung using RIPK1 antibody (22-922) at dilution of 1:100 (40x lens). |
5th Image Caption |
Immunohistochemistry of paraffin-embedded mouse spleen using RIPK1 antibody (22-922) at dilution of 1:100 (40x lens). |
6th Image Caption |
Immunofluorescence analysis of C6 cells using RIPK1 antibody (22-922) at dilution of 1:100. Blue: DAPI for nuclear staining. |
7th Image Caption |
Immunofluorescence analysis of U-2 OS cells using RIPK1 antibody (22-922) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.