Vergleich

RIPK1 Antibody

ArtNr PRS-22-922-50uL
Hersteller ProSci
Menge 50 uL
Kategorie
Typ Antibody Primary
Format Liquid
Applikationen WB, IF, IP, IHC
Specific against Human, Mouse, Rat
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias RIPK1 Antibody: RIP, RIP1, RIP, Cell death protein RIP, RIP-1
Lieferbar
Shipping
blue ice or RT
By Research Area
Apoptosis, Cancer, Cell Cycle, Immunology, Signal Transduction
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 170-440 of human RIPK1 (NP_003795.2).
Applications
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:200
Positive Control 1
Jurkat
Positive Control 2
HT-1080
Positive Control 3
DU145
Positive Control 4
K-562
Positive Control 5
NIH/3T3
Positive Control 6
Mouse liver
Predicted Molecular Weight
Observed: 76kDa
Purification
Affinity purification
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
RIPK1
Ncbi Gene Id #
8737
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
RIPK1
Ncbi Official Full Name
receptor (TNFRSF)-interacting serine-threonine kinase 1
Ncbi Organism
Homo sapiens
Swissprot #
Q13546
1st Image Caption
Western blot analysis of extracts of various cell lines, using RIPK1 antibody (22-922) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
2nd Image Caption
Western blot analysis of extracts of various cell lines, using RIPK1 antibody (22-922) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
3rd Image Caption
Immunohistochemistry of paraffin-embedded human tonsil using RIPK1 antibody (22-922) at dilution of 1:100 (40x lens).
4th Image Caption
Immunohistochemistry of paraffin-embedded rat lung using RIPK1 antibody (22-922) at dilution of 1:100 (40x lens).
5th Image Caption
Immunohistochemistry of paraffin-embedded mouse spleen using RIPK1 antibody (22-922) at dilution of 1:100 (40x lens).
6th Image Caption
Immunofluorescence analysis of C6 cells using RIPK1 antibody (22-922) at dilution of 1:100. Blue: DAPI for nuclear staining.
7th Image Caption
Immunofluorescence analysis of U-2 OS cells using RIPK1 antibody (22-922) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 uL
Lieferbar: In stock
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