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Beclin 1 Antibody, KO Validated

Beclin 1 Antibody, KO Validated

ArtNr	PRS-22-886-50uL
Hersteller	ProSci
Menge	50 uL
Kategorie	Neuroscience Neural Lineage Markers Antibodies Cell Biology Cell Status Necrosis Immunohistochemistry Immunoprecipitation Western Blot Immunofluorescence Autophagy Primary Antibodies
Typ	Antibody Primary
Format	Liquid
Applikationen	WB, IF, IP, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Beclin-1 Antibody: ATG6, VPS30, beclin1, GT197, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein
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Shipping	blue ice or RT
By Research Area	Apoptosis, Autophagy, Cancer, Cell Cycle, Signal Transduction
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Beclin 1 (NP_003757.1).
Applications	WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200 IP: 1:50 - 1:100
Positive Control 1	U-87MG
Positive Control 2	HeLa
Positive Control 3	MCF7
Positive Control 4	Mouse spleen
Positive Control 5	Rat ovary
Positive Control 6	Rat lung
Predicted Molecular Weight	Observed: 60kDa
Validation	Antibody is Knockout validated.
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	BECN1
Ncbi Gene Id #	8678
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	BECN1
Ncbi Official Full Name	beclin 1, autophagy related
Ncbi Organism	Homo sapiens
Swissprot #	Q14457
Background	This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.
1st Image Caption	Western blot analysis of extracts of various cell lines, using Beclin 1 antibody (22-886) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
2nd Image Caption	Western blot analysis of extracts from normal (control) and Beclin 1 knockout (KO) 293T cells, using Beclin 1 antibody (22-886) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
3rd Image Caption	Immunohistochemistry of paraffin-embedded human breast cancer using Beclin 1 antibody (22-886) at dilution of 1:100 (40x lens).
4th Image Caption	Immunohistochemistry of paraffin-embedded human stomach using Beclin 1 antibody (22-886) at dilution of 1:100 (40x lens).
5th Image Caption	Immunofluorescence analysis of HeLa cells using Beclin 1 antibody (22-886) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
6th Image Caption	Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug Beclin 1 antibody (22-886). Western blot was performed from the immunoprecipitate using Beclin 1 antibody (22-886) at a dilition of 1:1000.

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