ArtNr |
PRS-22-886-50uL |
Hersteller |
ProSci
|
Menge |
50 uL |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, IF, IP, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Beclin-1 Antibody: ATG6, VPS30, beclin1, GT197, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Apoptosis, Autophagy, Cancer, Cell Cycle, Signal Transduction |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Beclin 1 (NP_003757.1). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:100 |
Positive Control 1 |
U-87MG |
Positive Control 2 |
HeLa |
Positive Control 3 |
MCF7 |
Positive Control 4 |
Mouse spleen |
Positive Control 5 |
Rat ovary |
Positive Control 6 |
Rat lung |
Predicted Molecular Weight |
Observed: 60kDa |
Validation |
Antibody is Knockout validated. |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
BECN1 |
Ncbi Gene Id # |
8678 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
BECN1 |
Ncbi Official Full Name |
beclin 1, autophagy related |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q14457 |
Background |
This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using Beclin 1 antibody (22-886) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s. |
2nd Image Caption |
Western blot analysis of extracts from normal (control) and Beclin 1 knockout (KO) 293T cells, using Beclin 1 antibody (22-886) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s. |
3rd Image Caption |
Immunohistochemistry of paraffin-embedded human breast cancer using Beclin 1 antibody (22-886) at dilution of 1:100 (40x lens). |
4th Image Caption |
Immunohistochemistry of paraffin-embedded human stomach using Beclin 1 antibody (22-886) at dilution of 1:100 (40x lens). |
5th Image Caption |
Immunofluorescence analysis of HeLa cells using Beclin 1 antibody (22-886) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
6th Image Caption |
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug Beclin 1 antibody (22-886). Western blot was performed from the immunoprecipitate using Beclin 1 antibody (22-886) at a dilition of 1:1000. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.