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PPL Antibody

PPL Antibody

ArtNr	PRS-22-580-50uL
Hersteller	ProSci
Menge	50 uL
Kategorie	Oncology Antibodies Western Blot Immunofluorescence Primary Antibodies
Typ	Antibody Primary
Format	Liquid
Applikationen	WB, IF
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Periplakin, 190 kDa paraneoplastic pemphigus antigen, 195 kDa cornified envelope precursor protein, PPL, KIAA0568
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Shipping	blue ice or RT
By Research Area	Cell Cycle, Immunology, Signal Transduction
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human PPL (NP_002696.3).
Applications	WB: 1:500 - 1:2000 IF: 1:50 - 1:100
Positive Control 1	Mouse liver
Positive Control 2	Mouse lung
Positive Control 3	Mouse heart
Positive Control 4	Rat spinal cord
Predicted Molecular Weight	Observed: 205kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	PPL
Ncbi Gene Id #	5493
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	PPL
Ncbi Official Full Name	Periplakin
Ncbi Organism	Homo sapiens
Swissprot #	O60437
Background	The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling.
1st Image Caption	Western blot analysis of extracts of various cell lines, using PPL antibody (22-580) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
2nd Image Caption	Immunofluorescence analysis of MCF7 cells using PPL antibody (22-580). Blue: DAPI for nuclear staining.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 50 uL

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Lieferung vsl. bis 06.09.2024

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