ArtNr |
PRS-22-579-50uL |
Hersteller |
ProSci
|
Menge |
50 uL |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, IF, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
peptidylprolyl isomerase D (cyclophilin D), CYP-40, MGC33096, 40 kDa peptidyl-prolyl cis-trans isomerase D, PPIase, cyclophilin 40, cyclophilin D, cyclophilin-related protein, peptidylprolyl isomerase D, rotamase, CYP40, CYPD |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Immunology, Neuroscience |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 161-370 of human PPID (NP_005029.1). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100 |
Positive Control 1 |
HepG2 |
Positive Control 2 |
NIH/3T3 |
Positive Control 3 |
MCF7 |
Positive Control 4 |
U-87MG |
Positive Control 5 |
Mouse brain |
Positive Control 6 |
Rat brain |
Predicted Molecular Weight |
Observed: 41kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
PPID |
Ncbi Gene Id # |
5481 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
PPID |
Ncbi Official Full Name |
peptidylprolyl isomerase D |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q08752 |
Background |
The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant cyclosporin A. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using PPID antibody (22-579) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. |
2nd Image Caption |
Immunohistochemistry of paraffin-embedded rat brain using PPID Antibody (22-579) at dilution of 1:100 (40x lens). |
3rd Image Caption |
Immunohistochemistry of paraffin-embedded human kidney using PPID Antibody (22-579) at dilution of 1:100 (40x lens). |
4th Image Caption |
Immunohistochemistry of paraffin-embedded mouse brain using PPID Antibody (22-579) at dilution of 1:100 (40x lens). |
5th Image Caption |
Immunofluorescence analysis of A-549 cells using PPID antibody (22-579). Blue: DAPI for nuclear staining. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.