Vergleich

PPID Antibody

ArtNr PRS-22-579-50uL
Hersteller ProSci
Menge 50 uL
Kategorie
Typ Antibody Primary
Format Liquid
Applikationen WB, IF, IHC
Specific against Human, Mouse, Rat
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias peptidylprolyl isomerase D (cyclophilin D), CYP-40, MGC33096, 40 kDa peptidyl-prolyl cis-trans isomerase D, PPIase, cyclophilin 40, cyclophilin D, cyclophilin-related protein, peptidylprolyl isomerase D, rotamase, CYP40, CYPD
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Shipping
blue ice or RT
By Research Area
Immunology, Neuroscience
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 161-370 of human PPID (NP_005029.1).
Applications
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Positive Control 1
HepG2
Positive Control 2
NIH/3T3
Positive Control 3
MCF7
Positive Control 4
U-87MG
Positive Control 5
Mouse brain
Positive Control 6
Rat brain
Predicted Molecular Weight
Observed: 41kDa
Purification
Affinity purification
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
PPID
Ncbi Gene Id #
5481
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
PPID
Ncbi Official Full Name
peptidylprolyl isomerase D
Ncbi Organism
Homo sapiens
Swissprot #
Q08752
Background
The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant cyclosporin A.
1st Image Caption
Western blot analysis of extracts of various cell lines, using PPID antibody (22-579) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
2nd Image Caption
Immunohistochemistry of paraffin-embedded rat brain using PPID Antibody (22-579) at dilution of 1:100 (40x lens).
3rd Image Caption
Immunohistochemistry of paraffin-embedded human kidney using PPID Antibody (22-579) at dilution of 1:100 (40x lens).
4th Image Caption
Immunohistochemistry of paraffin-embedded mouse brain using PPID Antibody (22-579) at dilution of 1:100 (40x lens).
5th Image Caption
Immunofluorescence analysis of A-549 cells using PPID antibody (22-579). Blue: DAPI for nuclear staining.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 uL
Lieferbar: In stock
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