ArtNr |
PRS-22-295-50uL |
Hersteller |
ProSci
|
Menge |
50 uL |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, IF, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Calumenin, Crocalbin, IEF SSP 9302, CALU |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Neuroscience, Signal Transduction |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 20-315 of human CALU (NP_001210.1). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:200 |
Positive Control 1 |
NCI-H460 |
Positive Control 2 |
Mouse lung |
Predicted Molecular Weight |
Observed: 50kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
CALU |
Ncbi Gene Id # |
813 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
CALU |
Ncbi Official Full Name |
Calumenin |
Ncbi Organism |
Homo sapiens |
Swissprot # |
O43852 |
Background |
The product of this gene is a calcium-binding protein localized in the endoplasmic reticulum (ER) and it is involved in such ER functions as protein folding and sorting. This protein belongs to a family of multiple EF-hand proteins (CERC) that include reticulocalbin, ERC-55, and Cab45 and the product of this gene. Alternatively spliced transcript variants encoding different isoforms have been identified. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using CALU antibody (22-295) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 90s. |
2nd Image Caption |
Immunohistochemistry of paraffin-embedded mouse skin using CALU antibody (22-295) at dilution of 1:100 (40x lens). |
3rd Image Caption |
Immunohistochemistry of paraffin-embedded rat lung using CALU antibody (22-295) at dilution of 1:100 (40x lens). |
4th Image Caption |
Immunohistochemistry of paraffin-embedded mouse lung using CALU antibody (22-295) at dilution of 1:100 (40x lens). |
5th Image Caption |
Immunofluorescence analysis of NIH-3T3 cells using CALU antibody (22-295) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.