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HAO1 Antibody

ArtNr PRS-22-249-50uL
Hersteller ProSci
Menge 50 uL
Kategorie
Typ Antibody Primary
Format Liquid
Applikationen WB, IF, IHC
Specific against Human, Mouse, Rat
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias HAO1, GOX, GOX1, HAOX1
Lieferbar
Shipping
blue ice or RT
By Research Area
Signal Transduction
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human HAO1 (NP_060015.1).
Applications
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Positive Control 1
Mouse liver
Positive Control 2
Rat liver
Predicted Molecular Weight
Observed: 41kDa
Purification
Affinity purification
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
HAO1
Ncbi Gene Id #
54363
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
HAO1
Ncbi Official Full Name
hydroxyacid oxidase (glycolate oxidase) 1
Ncbi Organism
Homo sapiens
Swissprot #
Q9UJM8
Background
This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site.
1st Image Caption
Western blot analysis of extracts of various cell lines, using HAO1 antibody (22-249) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
2nd Image Caption
Immunohistochemistry of paraffin-embedded human liver cancer using HAO1 antibody (22-249) at dilution of 1:200 (40x lens).

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 uL
Lieferbar: In stock
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