ArtNr |
PRS-22-095-50uL |
Hersteller |
ProSci
|
Menge |
50 uL |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, IF, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
HSP90B1, ECGP, GP96, TRA1, GRP94, HEL35, HEL-S-125m |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Apoptosis, Cancer, Cell Cycle, Signal Transduction |
Immunogen |
A synthetic peptide corresponding to a sequence within amino acids 550-650 of human HSP90B1 (NP_003290.1). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200 |
Positive Control 1 |
HepG2 |
Positive Control 2 |
Jurkat |
Positive Control 3 |
HeLa |
Positive Control 4 |
NIH/3T3 |
Predicted Molecular Weight |
Observed: 95kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
HSP90B1 |
Ncbi Gene Id # |
7184 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
HSP90B1 |
Ncbi Official Full Name |
heat shock protein 90kDa beta (Grp94), member 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P14625 |
Background |
This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using HSP90B1 antibody (22-095). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
2nd Image Caption |
Immunofluorescence analysis of NIH-3T3 cells using HSP90B1 Polyclonal Antibody (22-095) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.