ArtNr |
PRS-22-051-50uL |
Hersteller |
ProSci
|
Menge |
50 uL |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, IF, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
COMT, catechol-O-methyltransferase, HEL-S-98n |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Neuroscience, Signal Transduction |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 42-221 of human COMT (NP_009294.1). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200 |
Positive Control 1 |
MCF7 |
Positive Control 2 |
HT-29 |
Positive Control 3 |
THP-1 |
Positive Control 4 |
A-431 |
Positive Control 5 |
HepG2 |
Positive Control 6 |
Mouse liver |
Predicted Molecular Weight |
Observed: 28-32kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
COMT |
Ncbi Gene Id # |
1312 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
COMT |
Ncbi Official Full Name |
catechol-O-methyltransferase |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P21964 |
Background |
Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using COMT antibody (22-051) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s. |
2nd Image Caption |
Immunohistochemistry of paraffin-embedded rat kidney using COMT antibody (22-051) at dilution of 1:100 (40x lens). |
3rd Image Caption |
Immunohistochemistry of paraffin-embedded mouse kidney using COMT antibody (22-051) at dilution of 1:100 (40x lens). |
4th Image Caption |
Immunofluorescence analysis of U2OS cells using COMT antibody (22-051). Blue: DAPI for nuclear staining. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.