Vergleich

COMT Antibody

ArtNr PRS-22-051-50uL
Hersteller ProSci
Menge 50 uL
Kategorie
Typ Antibody Primary
Format Liquid
Applikationen WB, IF, IHC
Specific against Human, Mouse, Rat
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias COMT, catechol-O-methyltransferase, HEL-S-98n
Lieferbar
Shipping
blue ice or RT
By Research Area
Neuroscience, Signal Transduction
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 42-221 of human COMT (NP_009294.1).
Applications
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Positive Control 1
MCF7
Positive Control 2
HT-29
Positive Control 3
THP-1
Positive Control 4
A-431
Positive Control 5
HepG2
Positive Control 6
Mouse liver
Predicted Molecular Weight
Observed: 28-32kDa
Purification
Affinity purification
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
COMT
Ncbi Gene Id #
1312
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
COMT
Ncbi Official Full Name
catechol-O-methyltransferase
Ncbi Organism
Homo sapiens
Swissprot #
P21964
Background
Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.
1st Image Caption
Western blot analysis of extracts of various cell lines, using COMT antibody (22-051) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
2nd Image Caption
Immunohistochemistry of paraffin-embedded rat kidney using COMT antibody (22-051) at dilution of 1:100 (40x lens).
3rd Image Caption
Immunohistochemistry of paraffin-embedded mouse kidney using COMT antibody (22-051) at dilution of 1:100 (40x lens).
4th Image Caption
Immunofluorescence analysis of U2OS cells using COMT antibody (22-051). Blue: DAPI for nuclear staining.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 uL
Lieferbar: In stock
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