ArtNr |
PRS-2136-0.1mg |
Hersteller |
ProSci
|
Menge |
0.1 mg |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Liquid |
Applikationen |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
APP Antibody: AAA, AD1, PN2, ABPP, APPI, CVAP, ABETA, PN-II, CTFgamma, A4, Amyloid beta A4 protein, APP |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Neuroscience |
Homology |
Predicted species reactivity based on immunogen sequence: Pig: (100%), Guinea pig: (100%) |
Immunogen |
APP antibody was raised against a 15 amino acid peptide near the carboxy terminus of human APP.
The immunogen is located within the last 50 amino acids of APP. |
Applications |
APP antibody can be used for detection of APP and the C99 fragment by Western blot at 0.5 - 1 μ g/mL. Antibody can also be used for immunohistochemistry starting at 2 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested. |
Positive Control 1 |
Cat. No. 1403 - Mouse Brain Tissue Lysate |
Predicted Molecular Weight |
Predicted: 83 kDa
Observed: 110 kDa |
Purification |
APP Antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
APP Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/ml |
Storage Conditions |
APP antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Related Products |
Cat. No. 2136P – APP Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
APP |
Accession # |
CAA30050 |
Protein Gi # |
28721 |
Ncbi Gene Id # |
351 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
APP |
Ncbi Official Full Name |
amyloid beta (A4) precursor protein |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P05067 |
Background |
APP Antibody: Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The beta-amyloid protein precursor (APP) is cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the brains of all suffers of Alzheimer's disease. |
Background References 1 |
Ponte P, Gonzalez-DeWhitt P, Schilling J, Miller J, Hsu D, Greenberg B, Davis K, Wallace W, Lieberburg I, Fuller F. A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. Nature 1988; 331:525-7 |
Background References 2 |
Selkoe D.J. Cell biology of the amyloid β -protein precursor and the mechanism of Alzheimer's disease.Annu Rev Cell Biol 1994; 10:373-403 (RD1299) |
1st Image Caption |
Western blot analysis of APP in mouse brain tissue lysate with APP antibody at (A) 0.5 and (B) 1 μ g/ml. |
2nd Image Caption |
Immunohistochemical staining of rat brain using APP antibody at 2 μ g/mL. |
3rd Image Caption |
Immunofluorescence of APP in Rat Brain cells with APP antibody at 20 μ g/mL. |
4th Image Caption |
Immunohistochemistry of APP in mouse brain tissue with APP Antibody at 5 μ g/mL. |
5th Image Caption |
Immunofluorescence of APP in mouse brain tissue with APP Antibody at 20 μ g/mL.
Green: APP antibody (2136) Red: Phylloidin staining Blue: DAPI staining |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.