Vergleich

FBXL5 Antibody

ArtNr PRS-19-751-50uL
Hersteller ProSci
Menge 50 uL
Kategorie
Typ Antibody Primary
Format Liquid
Applikationen WB, IHC
Specific against Human, Mouse, Rat
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias FBXL5, FBL4, FBL5, FLR1
Lieferbar
Shipping
blue ice or RT
By Research Area
Cell Cycle
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human FBXL5 (NP_036293.1).
Applications
WB: 1:500 - 1:1000

IHC: 1:50 - 1:100
Positive Control 1
293T
Positive Control 2
Mouse eye
Positive Control 3
Mouse brain
Positive Control 4
Mouse lung
Positive Control 5
Rat testis
Positive Control 6
Rat lung
Predicted Molecular Weight
Observed: 79kDa
Purification
Affinity purification
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
FBXL5
Ncbi Gene Id #
26234
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
FBXL5
Ncbi Official Full Name
F-box and leucine-rich repeat protein 5
Ncbi Organism
Homo sapiens
Swissprot #
Q9UKA1
Background
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains several tandem leucine-rich repeats. Alternatively spliced transcript variants have been described for this locus.
1st Image Caption
Western blot analysis of extracts of various cell lines, using FBXL5 antibody (19-751) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
2nd Image Caption
Immunohistochemistry of paraffin-embedded human stomach using FBXL5 antibody (19-751) at dilution of 1:100 (40x lens).
3rd Image Caption
Immunohistochemistry of paraffin-embedded human colon carcinoma using FBXL5 antibody (19-751) at dilution of 1:100 (40x lens).

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 uL
Lieferbar: In stock
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