ArtNr |
PRS-19-528-100uL |
Hersteller |
ProSci
|
Menge |
100 uL |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
L45mt, MRP-L45, 39S ribosomal protein L45, mitochondrial, mitochondrial large ribosomal subunit protein mL45 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Signal Transduction |
Immunogen |
A synthetic peptide of human MRPL45 |
Applications |
WB: 1:500 - 1:2000 |
Positive Control 1 |
HepG2 |
Positive Control 2 |
HeLa |
Positive Control 3 |
U-87MG |
Predicted Molecular Weight |
Observed: 35kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, pH7.3. |
Storage Conditions |
Store at 4˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
MRPL45 |
Ncbi Gene Id # |
84311 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
MRPL45 |
Ncbi Official Full Name |
mitochondrial ribosomal protein L45 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q9BRJ2 |
Background |
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 2p and 17q. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using MRPL45 antibody (19-528). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.