ArtNr |
PRS-19-029-50uL |
Hersteller |
ProSci
|
Menge |
50 uL |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, IF, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
MAP1B, FUTSCH, MAP-1B, MAP5 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cell Cycle, Neuroscience, Signal Transduction |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 1820-2100 of human MAP1B (NP_005900.2). |
Applications |
WB: 1:200 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200 |
Positive Control 1 |
DU145 |
Positive Control 2 |
293T |
Positive Control 3 |
HT-1080 |
Positive Control 4 |
Rat testis |
Predicted Molecular Weight |
Observed: 350kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
MAP1B |
Ncbi Gene Id # |
4131 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
MAP1B |
Ncbi Official Full Name |
microtubule-associated protein 1B |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P46821 |
Background |
This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The product of this gene is a precursor polypeptide that presumably undergoes proteolytic processing to generate the final MAP1B heavy chain and LC1 light chain. Gene knockout studies of the mouse microtubule-associated protein 1B gene suggested an important role in development and function of the nervous system. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using MAP1B antibody (19-029) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. |
2nd Image Caption |
Immunofluorescence analysis of C6 cells using MAP1B antibody (19-029) at dilution of 1:100. Blue: DAPI for nuclear staining. |
3rd Image Caption |
Immunofluorescence analysis of NIH/3T3 cells using MAP1B antibody (19-029) at dilution of 1:100. Blue: DAPI for nuclear staining. |
4th Image Caption |
Immunofluorescence analysis of U2OS cells using MAP1B antibody (19-029) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.