Vergleich

KRT5 Antibody

ArtNr PRS-18-754-50uL
Hersteller ProSci
Menge 50 uL
Kategorie
Typ Antibody Primary
Format Liquid
Applikationen WB, IF, IHC
Specific against Human, Mouse
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias KRT5, 58 kd cytokeratin, 58 kDa cytokeratin, CK5, CK-5, Cytokeratin-5, DDD, EBS2, Keratin 5, KRT5A, Type-II keratin Kb5, Cytokeratin 5, K5, Keratin-5
Lieferbar
Shipping
blue ice or RT
By Research Area
Cell Cycle, Signal Transduction
Immunogen
Recombinant protein of human KRT5
Applications
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Positive Control 1
Mouse kidney
Positive Control 2
Mouse thymus
Positive Control 3
Mouse skeletal muscle
Predicted Molecular Weight
Observed: 70kDa
Purification
Affinity purification
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
KRT5
Ncbi Gene Id #
3852
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol
KRT5
Ncbi Official Full Name
keratin 5
Ncbi Organism
Homo sapiens
Swissprot #
P13647
Background
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
1st Image Caption
Western blot analysis of extracts of various cell lines, using KRT5 antibody (18-754) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
2nd Image Caption
Immunohistochemistry of paraffin-embedded human kidney using KRT5 Antibody (18-754) at dilution of 1:100 (40x lens).
3rd Image Caption
Immunohistochemistry of paraffin-embedded human uterine cancer using KRT5 Antibody (18-754) at dilution of 1:100 (40x lens).
4th Image Caption
Immunofluorescence analysis of HeLa cells using KRT5 antibody (18-754).

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 uL
Lieferbar: In stock
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