ArtNr |
PRS-18-754-50uL |
Hersteller |
ProSci
|
Menge |
50 uL |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, IF, IHC |
Specific against |
Human, Mouse |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
KRT5, 58 kd cytokeratin, 58 kDa cytokeratin, CK5, CK-5, Cytokeratin-5, DDD, EBS2, Keratin 5, KRT5A, Type-II keratin Kb5, Cytokeratin 5, K5, Keratin-5 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cell Cycle, Signal Transduction |
Immunogen |
Recombinant protein of human KRT5 |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200 |
Positive Control 1 |
Mouse kidney |
Positive Control 2 |
Mouse thymus |
Positive Control 3 |
Mouse skeletal muscle |
Predicted Molecular Weight |
Observed: 70kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
KRT5 |
Ncbi Gene Id # |
3852 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
KRT5 |
Ncbi Official Full Name |
keratin 5 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P13647 |
Background |
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using KRT5 antibody (18-754) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s. |
2nd Image Caption |
Immunohistochemistry of paraffin-embedded human kidney using KRT5 Antibody (18-754) at dilution of 1:100 (40x lens). |
3rd Image Caption |
Immunohistochemistry of paraffin-embedded human uterine cancer using KRT5 Antibody (18-754) at dilution of 1:100 (40x lens). |
4th Image Caption |
Immunofluorescence analysis of HeLa cells using KRT5 antibody (18-754). |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.