ArtNr |
PRS-18-717-50uL |
Hersteller |
ProSci
|
Menge |
50 uL |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB |
Specific against |
Human, Mouse |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
PDCD4 Antibody: H731, H731, Programmed cell death protein 4, Neoplastic transformation inhibitor protein |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Apoptosis, Cancer, Cell Cycle, Signal Transduction |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PDCD4 (NP_055271.2). |
Applications |
WB: 1:500 - 1:2000 |
Positive Control 1 |
MCF-7 |
Positive Control 2 |
HeLa |
Positive Control 3 |
HepG2 |
Positive Control 4 |
Mouse thymus |
Predicted Molecular Weight |
Observed: 60kDa |
Validation |
Antibody is Knockout validated. |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
PDCD4 |
Ncbi Gene Id # |
27250 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
PDCD4 |
Ncbi Official Full Name |
programmed cell death 4 (neoplastic transformation inhibitor) |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q53EL6 |
Background |
This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using PDCD4 antibody (18-717) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s. |
2nd Image Caption |
Western blot analysis of extracts from normal (control) and PDCD4 knockout (KO) HeLa cells, using PDCD4 antibody (18-717) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.