ArtNr |
PRS-18-320-50uL |
Hersteller |
ProSci
|
Menge |
50 uL |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, IF, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
PSAP, Cerebroside sulfate activator, GLBA, Proactivator polypeptide, Prosaposin, SAP1 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Signal Transduction |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 60-310 of human PSAP (NP_002769.1). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200 |
Positive Control 1 |
A-549 |
Positive Control 2 |
SKOV3 |
Positive Control 3 |
293T |
Predicted Molecular Weight |
Observed: 58kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
PSAP |
Ncbi Gene Id # |
5660 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
PSAP |
Ncbi Official Full Name |
prosaposin |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P07602 |
Background |
This gene encodes a highly conserved preproprotein that is proteolytically processed to generate four main cleavage products including saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using PSAP antibody (18-320) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5s. |
2nd Image Caption |
Immunohistochemistry of paraffin-embedded rat brain using PSAP antibody (18-320) at dilution of 1:200 (40x lens). |
3rd Image Caption |
Immunohistochemistry of paraffin-embedded rat spleen using PSAP antibody (18-320) at dilution of 1:200 (40x lens). |
4th Image Caption |
Immunohistochemistry of paraffin-embedded mouse brain using PSAP antibody (18-320) at dilution of 1:200 (40x lens). |
5th Image Caption |
Immunofluorescence analysis of MCF-7 cells using PSAP antibody (18-320). Blue: DAPI for nuclear staining. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.