ArtNr |
PRS-18-270-50uL |
Hersteller |
ProSci
|
Menge |
50 uL |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, IF, IHC |
Specific against |
Human, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
PTPN2, PTPT, TC-PTP, TCELLPTP, TCPTP, PTN2 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Immunology, Signal Transduction |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-353 of human PTPN2 (NP_536348.1). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200 |
Positive Control 1 |
SW480 |
Positive Control 2 |
BT-474 |
Positive Control 3 |
Jurkat |
Positive Control 4 |
K-562 |
Predicted Molecular Weight |
Observed: 45kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
PTPN2 |
Ncbi Gene Id # |
5771 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
PTPN2 |
Ncbi Official Full Name |
protein tyrosine phosphatase, non-receptor type 2 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P17706 |
Background |
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Multiple alternatively spliced transcript variants encoding different isoforms have been found. Two highly related but distinctly processed pseudogenes that localize to chromosomes 1 and 13, respectively, have been reported. |
1st Image Caption |
Western blot analysis of extracts of various cell lines, using PTPN2 antibody (18-270) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. |
2nd Image Caption |
Immunohistochemistry of paraffin-embedded rat heart using PTPN2 antibody (18-270) at dilution of 1:100 (40x lens). |
3rd Image Caption |
Immunofluorescence analysis of MCF-7 cells using PTPN2 antibody (18-270). Blue: DAPI for nuclear staining. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.