ArtNr |
PRS-16-869-50uL |
Hersteller |
ProSci
|
Menge |
50 uL |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Liquid |
Applikationen |
WB, IF, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
PIK3R3, DKFZp686P05226, FLJ41892, p55-GAMMA, p55 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Apoptosis, Cell Cycle, Immunology, Signal Transduction |
Immunogen |
Recombinant protein of human PIK3R3. |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100 |
Positive Control 1 |
Mouse brain |
Predicted Molecular Weight |
Observed: 54kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
PIK3R3 |
Ncbi Gene Id # |
8503 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
PIK3R3 |
Ncbi Official Full Name |
phosphoinositide-3-kinase, regulatory subunit 3 (gamma) |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q92569 |
Background |
Phosphatidylinositol 3-kinase (PI3K) phosphorylates phosphatidylinositol and similar compounds, which then serve as second messengers in growth signaling pathways. PI3K is composed of a catalytic and a regulatory subunit. The protein encoded by this gene represents a regulatory subunit of PI3K. The encoded protein contains two SH2 domains through which it binds activated protein tyrosine kinases to regulate their activity. |
1st Image Caption |
Western blot analysis of extracts of Mouse brain, using PIK3R3 antibody (16-869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |
2nd Image Caption |
Immunofluorescence analysis of NIH-3T3 cells using PIK3R3 antibody (16-869) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.